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DTprime II Real-Time PCR Systems (RUO)

New

The actual product may vary from the images shown on the website.

The new generation of DTprime II Real-Time PCR Systems has improved characteristics and expanded functionality. DTprime II are designed for qualitative and quantitative PCR: pathogen detection, genotyping, microbiome analysis, gene expression without requiring ROX as a passive reference dye, high-resolution melting curve (HRM), FRET, LAMP, etc. For research use only.

Safety, reliability and high precision of analysis

•  High uniformity of well heating ensures excellent reproducibility of results.

•  The hot lid adapts to a wide range of plastic due to inbuilt tube height auto measuring function.

•  7” color touchscreen is responsive with gloves.

•  Built-in operating system allows to use the instrument in stand-alone mode for simple tasks (uploading, choosing, running and saving protocols) and configure remote access by Ethernet.

•  Expanded connectivity – by Ethernet (local network, remote server, cloud storage), internal memory and USB.

•  Protection from mains voltage failures: the instrument is automatically continues the amplification program after power is restored.

•  If communication with the control PC is interrupted, DTprime II continue operates in autonomous mode.

•  The new 6-channel optical system with digital filter eliminates the overlap of signals between channels.

Best solution for high-flow labs

•   Available in 96- and 384-well formats.

•  High speed of temperature change (heating 5 °С/s, cooling 3.6 °С/s) decreases the time of amplification program.

•  New optical system free of regular calibration requirements.

•  Multiplex PCR (4, 5 or 6 optical channels).

•  DTprime II instruments are fully integrated into DTstream liquid handling automated process.

•  Software supports connection of several DT instruments to one PC in multi-window mode, data exchange with LIS, working with rdml, ect.

•  Ready PCR protocols and automatic assay report for DNA-Technology kits.

•  The compact design of the instrument saves space in the lab.

More features for research labs

•  Assay optimization with 2 directions thermal gradient: by rows and by columns (*M1 version).

• 3 or 6 independent temperature zones (*M3, *M6 version).

• 4, 5 or 6 optical channels.

•  Multiplex analysis with FRET probes (FAM-ROX).

•  Support melting curve and HRM (high resolution melt) analysis.

•  Flexible setting of test parameters for research.

Multifunctional DTmaster Software to analyze the results

Click here to download

 

Instrument

DTprime II

Sample capacity 96-well 96-well 96-well 384-well
Version *M1 *M3 *M6 *X1
Heat block type single block 3 sections 6 sections single block
Temperature gradient in 2 directions yes - - -
Maximum value of the gradient, °С 10 - - -
Maximum temperature difference between sections - ±10 ±10 -
Temperature increment yes -
Time increment yes -
Compatible consumables 200 μl tubes (individual or strips), 96-well plates 384-well plates
PCR-mix volume (recommended), μl 10-100 (10-50) 5-20
Built-in barcode scanner - yes
*Number of detection channels 4, 5 or 6
Heat block method Peltier
Temperature range, °С 0-100
Temperature accuracy, °С ±0,2
Temperature uniformity, °С 0.3
Maximum/ average heating rate, °С/s 5.0 / 4.0
Maximum/ average cooling rate, °С/s 3.6 / 2.5
Excitation source LED
Range of excitation wavelengths, nm FAM 450-470, HEX 520-540, ROX 565-595, Cy5 620-640, Cy5,5 677-697, FRET 450-470
Detection source CMOS
Range of emission wavelengths, nm FAM 500-530, HEX 550-570, ROX 605-635, Cy5 650-670, Cy5,5 716-746, FRET 605-635
Dimensions (WxDxH), mm 210x538x540
Weight, kg 28.4
MAX power consumption, W 1100
Automatic resumption of amplification program after voltage recovery yes
Remote access by Internet yes
Local network connection yes
Color touch panel 7” yes
Tube height measurement function yes
Warranty – 24 months yes
Result export formats rdml, xml, csv, txt, png
Absolute and relative quantitative analysis yes
Melting curve analysis yes
LAMP yes
Multiplex PCR (5 targets in one tube) yes
HRM yes

 

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