AZF Microdeletions

According to WHO, every year about 15 % of married couples seek medical help for infertility. Infertility is the inability of a man or woman of childbearing age to reproduce an offspring. A couple is considered infertile if the woman cannot get pregnant within one year of regular unprotected sex (at least once per week).

Male infertility is inability of a man to impregnate a woman. Genetic disorders are present in 30-50% of cases where oligozoospermia, azoospermia and other severe disorders are detected via semen analysis (baseline study in a barren marriage). Y-chromosome deletion in the AZF (azoospermia factor) region is the most common genetic factor of male infertility.

Y-chromosome microdeletions are not detected via cytogenetic analysis, thus making their molecular genetic search reasonable. The European Academy of Andrology (EAA) recommends testing all men with azoospermia and severe oligozoospermia (< 5 million sperm per milliliter of semen) for the presence of AZF deletion.

DNA-Technology developed a kit (see Tables 37-39) for detecting AZF deletions associated with male infertility. The analytical panel included 13 nonpolymorphic markers that allow detecting deletions in all AZF loci.

The AZF Microdeletions REAL-TIME PCR Genotyping Kit is an in vitro Nucleic Acid Test (NAT) – human genotyping-based product.

The AZF Microdeletions REAL-TIME PCR Genotyping Kit is designed to detect AZF locus deletions which are the common cause of male infertility defined by loss of spermatozoids motion ability by real-time PCR.

The AZF Microdeletions REAL-TIME PCR Genotyping Kit can be used only for men.

The AZF Microdeletions REAL-TIME PCR Genotyping Kit can be used in clinical and diagnostic laboratories of medical institutions and research practice.

Certification: RU/IVD