AZF Microdeletions

According to WHO, every year about 15 % of married couples seek medical help for infertility. Infertility is the inability of a man or woman of childbearing age to reproduce an offspring. A couple is considered infertile if the woman cannot get pregnant within one year of regular unprotected sex (at least once per week).

Male infertility is inability of a man to impregnate a woman. Genetic disorders are present in 30-50% of cases where oligozoospermia, azoospermia and other severe disorders are detected via semen analysis (baseline study in a barren marriage). Y-chromosome deletion in the AZF (azoospermia factor) region is the most common genetic factor of male infertility.

Y-chromosome microdeletions are not detected via cytogenetic analysis, thus making their molecular genetic search reasonable. The European Academy of Andrology (EAA) recommends testing all men with azoospermia and severe oligozoospermia (< 5 million sperm per milliliter of semen) for the presence of AZF deletion.

DNA-Technology developed a kit (see Tables 37-39) for detecting AZF deletions associated with male infertility. The analytical panel included 13 nonpolymorphic markers that allow detecting deletions in all AZF loci.

AZF Microdeletions REAL-TIME PCR Genotyping Kit is intended for detection of AZF locus deletions, which are the common cause of male infertility defined by loss of spermatozoids motion ability (azoospermia).

AZF Microdeletions REAL-TIME PCR Genotyping Kit contains oligonucleotides capable for detection of Y chromosome deletions, which are associated with azoospermia, SRY gene indicating the gender and additional genomic target serving as sample intake control.

Sample: peripheral blood

DNA extraction: PREP-GS-GENETICS and PREP-RAPID-GENETICS kits

Certification: CE/IVD