DTprime II Real-Time PCR Systems (RUO)
The new generation of DTprime II Real-Time PCR Systems has improved characteristics and expanded functionality. DTprime II are designed for qualitative and quantitative PCR: pathogen detection, genotyping, microbiome analysis, gene expression without requiring ROX as a passive reference dye, high-resolution melting curve (HRM), FRET, LAMP, etc. For research use only.
Safety, reliability and high precision of analysis
• High uniformity of well heating ensures excellent reproducibility of results.
• The hot lid adapts to a wide range of plastic due to inbuilt tube height auto measuring function.
• 7” color touchscreen is responsive with gloves.
• Built-in operating system allows to use the instrument in stand-alone mode for simple tasks (uploading, choosing, running and saving protocols) and configure remote access by Ethernet.
• Expanded connectivity – by Ethernet (local network, remote server, cloud storage), internal memory and USB.
• Protection from mains voltage failures: the instrument is automatically continues the amplification program after power is restored.
• If communication with the control PC is interrupted, DTprime II continue operates in autonomous mode.
• The new 6-channel optical system with digital filter eliminates the overlap of signals between channels.
Best solution for high-flow labs
• Available in 96- and 384-well formats.
• High speed of temperature change (heating 5 °С/s, cooling 3.6 °С/s) decreases the time of amplification program.
• New optical system free of regular calibration requirements.
• Multiplex PCR (4, 5 or 6 optical channels).
• DTprime II instruments are fully integrated into DTstream liquid handling automated process.
• Software supports connection of several DT instruments to one PC in multi-window mode, data exchange with LIS, working with rdml, ect.
• Ready PCR protocols and automatic assay report for DNA-Technology kits.
• The compact design of the instrument saves space in the lab.
More features for research labs
• Assay optimization with 2 directions thermal gradient: by rows and by columns (*M1 version).
• 3 or 6 independent temperature zones (*M3, *M6 version).
• 4, 5 or 6 optical channels.
• Multiplex analysis with FRET probes (FAM-ROX).
• Support melting curve and HRM (high resolution melt) analysis.
• Flexible setting of test parameters for research.
Multifunctional DTmaster Software to analyze the results
Instrument |
DTprime II |
|||
Sample capacity | 96-well | 96-well | 96-well | 384-well |
Version | *M1 | *M3 | *M6 | *X1 |
Heat block type | single block | 3 sections | 6 sections | single block |
Temperature gradient in 2 directions | yes | - | - | - |
Maximum value of the gradient, °С | 10 | - | - | - |
Maximum temperature difference between sections | - | ±10 | ±10 | - |
Temperature increment | yes | - | ||
Time increment | yes | - | ||
Compatible consumables | 200 μl tubes (individual or strips), 96-well plates | 384-well plates | ||
PCR-mix volume (recommended), μl | 10-100 (10-50) | 5-20 | ||
Built-in barcode scanner | - | yes | ||
*Number of detection channels | 4, 5 or 6 | |||
Heat block method | Peltier | |||
Temperature range, °С | 0-100 | |||
Temperature accuracy, °С | ±0,2 | |||
Temperature uniformity, °С | 0.3 | |||
Maximum/ average heating rate, °С/s | 5.0 / 4.0 | |||
Maximum/ average cooling rate, °С/s | 3.6 / 2.5 | |||
Excitation source | LED | |||
Range of excitation wavelengths, nm | FAM 450-470, HEX 520-540, ROX 565-595, Cy5 620-640, Cy5,5 677-697, FRET 450-470 | |||
Detection source | CMOS | |||
Range of emission wavelengths, nm | FAM 500-530, HEX 550-570, ROX 605-635, Cy5 650-670, Cy5,5 716-746, FRET 605-635 | |||
Dimensions (WxDxH), mm | 210x538x540 | |||
Weight, kg | 28.4 | |||
MAX power consumption, W | 1100 | |||
Automatic resumption of amplification program after voltage recovery | yes | |||
Remote access by Internet | yes | |||
Local network connection | yes | |||
Color touch panel 7” | yes | |||
Tube height measurement function | yes | |||
Warranty – 24 months | yes | |||
Result export formats | rdml, xml, csv, txt, png | |||
Absolute and relative quantitative analysis | yes | |||
Melting curve analysis | yes | |||
LAMP | yes | |||
Multiplex PCR (5 targets in one tube) | yes | |||
HRM | yes |