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DTprime

The actual product may vary from the images shown on the website.

DTprime real-time PCR instrument is used for qualitative and quantitative analysis of DNA and RNA targets. DTprime is an optimum choice for diagnostic laboratories with high throughput workflow when performing routine analyses and for research organizations where optimization and fine-tuning adjustments are important. The instrument can be used for qualitative and quantitative analysis in the fields of:

• Human genetics;

• Gene Expression profiling;

• Pathogen detection, quantification and genotyping;

• Microbiome composition analysis.

The software DTmaster with user-friendly interface, common to all DT real-time PCR instruments, controls the instrument. Original DTmaster software lowers the probability of mistakes and is used for sophisticated assays requiring multiple settings application.

Advantages of DTprime:

• Tubes Height Measurement system provides "openness" of the instrument to different types of plastic (low and high profile; flat and domed caps; strips, plates).

• Simultaneous detection of a fluorescent signal in all wells of the matrix provides uniformity of detection and high speed of the instrument;

• Narrow range filters (cross-talk cut-off function);

• No "edge effect" - the thermo-block moving and positioning system ensures its movement in the horizontal and vertical positions, which guarantees complete isolation of the reaction tubes from the influence of environmental factors such as light penetration into the optical path and temperature non-uniformity between the edge rows of the matrix and its central part.

• The hot lid module provides reliable heat contact between tubes and the thermal block plate maintaining temperature of the tube caps at the level of 105±1°С in order to prevent condensate formation on the tube caps, since it may cause substantial distortions in the luminous flux measurements;

• Data protection system provides stable and safe operation of the instrument in the event of an unstable power supply. All parameters of the current run are recorded in the instrument's own memory;

• The software has a plenty of settings allowing advanced users to obtain specific data and finely tune instrument for a given task;

• Automated filling template in case of usage DNA-Technology PCR kits;

• Can be integrated into laboratory information systems (LIS) to store data in standard graphic and text formats;

• Ability to control multiple instruments from a single control computer increases lab productivity and allows you to simultaneously conduct a wide range of PCR tests using kits with different amplification programs;

• The narrow body and compact design allow you to place several instruments in a small area.

 

Типы детекции:

dimensions

210 × 540 × 540

weight

27 kg

Parameter

Real-time PCR DTprime instruments

DTprime 4М1

DTprime 5М1

DTprime 5М3

DTprime 5М6

DTprime 4Х1

DTprime 5Х1

General characteristics

Color display:

  • TFT 129х97 mm

  • 640х480 pix

  • Increased service life (at least 30 000 hours)

Built-in barcode scanner

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Hot lid temperature 105°С±1°С

Automatic test tube height measurement

Warranty period (24 months)

Post-warranty service

Line voltage 100-240 W

Power consumption max 550 W

Frequency 50/60 Hz

Size 210 × 540 × 540 mm

Mass 27 kg

Thermal unit characteristics

Thermal block plate format

Aluminum matrix

Heating and cooling method with 6 Peltier thermoelectric elements

Forced cooling and heating of matrix

No «edge effect»

Number of wells in thermal block

96

96

96

96

384

384

Volume of reaction mixture (allowable), mcl

10 - 100

10 - 100

10 - 100

10 - 100

5-30

5-30

Volume of reaction mixture (recommended), mcl

10-50

10-50

10-50

10-50

5-25

5-25

Consumables type (separate tubes, stripes, microplates)

0,2 ml

0,2 ml

0,2 ml

0,2 ml

384-well PCR plate 0,045 ml

3 384-well PCR plate 0,045 ml

Isolated pseudo blocks

Monoblock

Monoblock

3

6

Monoblock

Monoblock

Independent temperature control of pseudo blocks with time-synchronous profile

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Temperature gradient between pseudo blocks with independent

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0-8

0-8

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control, °С

Thermal block temperature range 0-100°С

Temperature accuracy ±0,2°С

Temperature uniformity ±0,15°С

Maximum heating rate of thermal block, °С/sec

3,5

3,5

3,5

3,5

2,5

2,5

Maximum cooling rate of thermal block, °С/sec

2,5

2,5

2,5

2,5

1,5

1,5

Average heating rate, °С/sec

3,3

3,3

3,3

3,3

2,1

2,1

Average cooling rate, °С/sec

2,1

2,1

2,1

2,1

1

1

Bidirectional gradient:8 vertical rows and 12 horizontal raws, ºС

Programmable range of bidirectional gradient 0 – 8 ºС

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Temperature step (0,1 ºС)

Time increment (discreteness 0,1 sec)

General characteristics of optical system

Excitation source - LED

Number of excitation sources

4

5

5

5

4

5

Spectral range, nm

470

530

580

630

470

530

580

630

687

470

530

580

630

687

470

530

580

630

687

470

530

580

630

470

530

580

630

687

CCD camera

CCD matrix

Number of fluorescence measuring channels

4

5

5

5

4

5

Detection for each channel, nm

515

560

620

660

515

560

620

660

731

515

560

620

660

731

515

560

620

660

731

515

560

620

660

515

560

620

660

731

Simultaneous signal detection in each well

Light filters (absolute isolation of the optical channel from external light)

Eliminated cross-channel effect

Scanning time for all channels is no more than 20 sec.

"Threshold" level of fluorescence 0,05х10Е-12М

Calibrated dyes (per channel)

Fam

Hex

Rox

Су5

Fam

Hex

Rox

Су5

Су5,5

Fam

Hex

Rox

Су5

Су5,5

Fam

Hex

Rox

Су5

Су5,5

Fam

Hex

Rox

Су5

Fam

Hex

Rox

Су5

Су5,5

Compatibility with other fluorophores:
    • Fam - SybrGreen
    • Hex - VIC, R6G
    • Rox - TAMRA

No calibrators / need to calibrate

Technology, tests

Dynamic range 9 lg

Melting curves analysis

Absolute and relative quantitative analysis

Software

USB-devices connection

Computer interface

(USB 2.0 high speed)

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Integration to LIS

Results export as .xml, .txt

Operating system: Windows® 7, Windows® 8, Windows® 10, Windows® XP, Windows®Vista

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