HLA-DRB1

The human leukocyte antigen (HLA) is a human histocompatibility gene system – a group of histocompatibility antigens. There are over 150 antigens. The locus is located on chromosome 6; it contains a large number of genes associated with the human immune system.

For diagnostic purposes HLA typing of the DRB1 gene at low resolution or at the level of 13 allele groups are the most commonly used. In some cases, it is also necessary to type HLA-DQA1 and HLA-DQB1 genes.

Genotyping the DRB1 locus at allele group level (low resolution) is used for selecting a tissue-compatible donor and recipient for the first-set grafting of organs, for instance kidneys. This level of genotyping is sufficient and meets the standards of the advanced transplantology centers throughout the world.

The risk of developing type 1 diabetes, which is the one of the most severe autoimmune diseases, is 10 times higher when either variant from the following genotype is determined: DRB1*01, *03,*04, *08, *09, *10.

HLA typing of spouses is used to diagnose causes of reproductive failures to identify similarities in the couple’s HLA gene variants.

HLA-DRB1 REAL-TIME PCR Genotyping Kit is intended for simultaneous detection of 13 alleles and groups of alleles of human major histocompatibility complex DRB1 gene by polymerase chain reaction.

Sample: peripheral blood

DNA extraction: PREP-GS-GENETICS and PREP-RAPID-GENETICS kits

Certification: RU/IVD