HLA-В27

The genetically caused seronegativity of some diseases make them inaccessible for analysis by standard serological methods, while PCR method gives a positive result, for instance, seronegative spondyloarthritis (SpA) – the disease is characterized by lesions of the sacroiliac joint with tendency to family aggregation. The SpA group includes 10 diseases associated with the presence of HLA-B27 – an antigen: idiopathic ankylosing spondylitis, psoriatic arthritis, Reiter’s syndrome, ulcerative colitis, Crohn’s disease, Whipple’s disease, juvenile chronic arthritis, reactive arthritis (Yersinia, Shigella, and Salmonella), acute anterior uveitis and Behcet’s syndrome.

HLA-B27 carriage detection is one of the most advanced approaches in early diagnosis and selection of treatment for a number of autoimmune diseases.

The DNA-Technology HLA-B27 REAL-TIME PCR Genotyping Kit is intended for rapid group-specific detection of HLA-B27 alleles (major histocompatibility complex, class I, B) by Real-Time PCR method in human biological samples (peripheral blood).

These alleles are generally recognized as a genetic marker of multiple disease conditions e.g. rheumatoid arthritis and ankylosing spondylitis (Bekhterev’s disease).

Indications for genetic HLA-B27 test:

• Necessity to eliminate a possibility of ankylosing spondylitis in a patient, whose relatives are suffering from this disease;

• Differential diagnosis of incomplete forms of Reiter’s syndrome (without urethritis or uveitis) with gonococcal arthritis;

• Differential diagnosis of Reiter’s syndrome, accompanied by severe arthritis and rheumatoid arthritis;

• When examining patients with juvenile rheumatoid arthritis. If the HLA-B27 antigen is not detected, ankylosing spondylitis and Reiter’s syndrome are unlikely, although these diseases cannot be completely excluded in this case.

Sample: peripheral blood

DNA extraction: PREP-GS-GENETICS and PREP-RAPID-GENETICS kits

Certification: CE IVD