HCV Genotyping

During diagnosis of blood-borne infections, PCR technology has a great importance especially in blood banking and to detection very early infections before antibodies have been developed.

PCR technology solves the following blood banking tasks:

•  Ensuring infection safety during the period of “seronegative window”;

•   Providing information that no infectious agents were found in the blood or blood components with ambiguous results of enzyme multiplied immunoassay obtained;

•  Identifying true virus carriers among seropositive individuals.

With PCR it is possible to detect the RNA of hepatitis C virus not only in blood serum but also in liver biopsy specimens, which is important in confirming the role of hepatitis C virus in the formation of hepatocellular carcinoma. In such patients, hepatitis C virus RNA is detected in hepatocytes and in the absence of anti-HCV and HCV RNA in blood serum. Anti-HCV does not appear at all among a number of patients with self-limited course of infection.

The HCV Real-time PCR genotyping Kit is intended for detection of Hepatitis C virus and its genotyping in samples of peripheral blood plasma by real-time PCR method.

The kit can be used for in vitro diagnostics of 1a, 1b, 2 and 3a/3b.

Sample: peripheral blood plasma

DNA extraction: PREP-NA DNA/RNA extraction kit

Sensitivity: not less than 200 copies of HCV RNA per 1.0 mL of blood plasma.

Certification: RU/IVD