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HLA-В27 alleles genotyping Kit

The actual product may vary from the images shown on the website.

The genetically caused seronegativity of some diseases make them inaccessible for analysis by standard serological methods, while PCR method gives a positive result, for instance, seronegative spondyloarthritis (SpA) – the disease is characterized by lesions of the sacroiliac joint with tendency to family aggregation. The SpA group includes 10 diseases associated with the presence of HLA-B27 – an antigen: idiopathic ankylosing spondylitis, psoriatic arthritis, Reiter’s syndrome, ulcerative colitis, Crohn’s disease, Whipple’s disease, juvenile chronic arthritis, reactive arthritis (Yersinia, Shigella, and Salmonella), acute anterior uveitis and Behcet’s syndrome.

HLA-B27 carriage detection is one of the most advanced approaches in early diagnosis and selection of treatment for a number of autoimmune diseases.

The DNA-Technology HLA-B27 REAL-TIME PCR Genotyping Kit is intended for rapid group-specific detection of HLA-B27 alleles (major histocompatibility complex, class I, B) by Real-Time PCR method in human biological samples (peripheral blood).

These alleles are generally recognized as a genetic marker of multiple disease conditions e.g. rheumatoid arthritis and ankylosing spondylitis (Bekhterev’s disease).

Indications for genetic HLA-B27 test:

• Necessity to eliminate a possibility of ankylosing spondylitis in a patient, whose relatives are suffering from this disease;

• Differential diagnosis of incomplete forms of Reiter’s syndrome (without urethritis or uveitis) with gonococcal arthritis;

• Differential diagnosis of Reiter’s syndrome, accompanied by severe arthritis and rheumatoid arthritis;

• When examining patients with juvenile rheumatoid arthritis. If the HLA-B27 antigen is not detected, ankylosing spondylitis and Reiter’s syndrome are unlikely, although these diseases cannot be completely excluded in this case.

Sample: peripheral blood

DNA extraction: PREP-GS-GENETICS and PREP-RAPID-GENETICS kits

Certification: RUO

Типы детекции:
Real-time format is designed to detect PCR results during amplification with the help of detecting amplifiers (in real time).
RT

Storage requirements

+2...+8° С

Except of minus part of the kit

-18 … -22° С

Shelf life

12 months

Detection instruments

Number of tests

Tubes(ml)/ Strips

Product number

Only DT instruments with 2 and more detection channels

48

strips 0.2x8

R1-H004-S3/4EU