Bordetella pertussis

According to WHO, respiratory tract infections are one of the main causes of sickness rate and mortality both at infancy and at older ages. About 70 % of these infections affect the upper respiratory tract and middle ear region.

Diagnosis and treatment of respiratory infections remain the focus of attention among both therapists and pediatricians since these infections account for over 90 % of all outpatients’ complaints, especially during autumn and winter seasons. 

Bordetella pertussis causes whooping cough (pertussis), an acute respiratory disease that is clinically manifested in the form of spasmodic coughing spells, infection of the respiratory, nervous and cardiovascular systems without primary intoxication and temperature reaction.

The “golden standard” for laboratory diagnosis of pertussis implies inoculation of nasopharyngeal secretions during the first 2-3 weeks of illness; later the test validity declines. Applying serological tests (antibodies to Bordetella pertussis antigens and Bordetella pertussis toxin) is reasonable at the later stages of infection; the test has high specificity but relatively low sensitivity.

The Bordetella pertussis Real-Time PCR Detection Kit is in vitro DNA test, which is intended for the specific identification of Bordetella pertussis in human biological samples.

Sample: scrapes from posterior pharyngeal wall

DNA extraction: PREP-NA, PREP-GS, PREP-RAPID extraction kits

Certification: RUO